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A Method for Cryopreserving Chicken Primordial Germ Cells

โœ Scribed by Moore, D. T.; Purdy, P. H.; Blackburn, H. D.


Book ID
121671068
Publisher
Poultry Science Association
Year
2006
Tongue
English
Weight
553 KB
Volume
85
Category
Article
ISSN
0032-5791

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Gonadal primordial germ cells (gPGCs) were collected from gonadal anlage of 5day-old White Leghorn (WL) embryos. Collected gPGCs were suspended in freezing medium containing 10% dimethyl sulphoxide (DMSO). The cell-suspension was frozen at 1ยฐC/min until the temperature reached -80ยฐC; cells were then