A method for concentration of nucleoside triphosphates by coprecipitation with calcium fluoride

✍ Scribed by Eila I. Kukko; Tuija M. Kallio; Jukka K. Heinonen

• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 279 KB
• Volume: 133
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(83)90221-x

✦ Synopsis

A method for concentration of nucleoside triphosphates (NTP) is described. NTPs are quantitatively coprecipitated from the solution with calcium fluoride. The precipitate is separated by filtration through a membrane filter and NTPs are dissolved from the filter by immersing it in 0.5 N H2SO4. With this method also nucleoside diphosphates can be efficiently concentrated, but the method does not work with nucleoside monophosphates or cyclic AMP.