A method for computing active drug from plasma levels in cases of multiple binding

✍ Scribed by Samuel Garten; Walter D. Wosilait

• Publisher: Elsevier Science
• Year: 1971
• Weight: 261 KB
• Volume: 2
• Category: Article
• ISSN: 0010-468X
• DOI: 10.1016/0010-468x(71)90008-0

✦ Synopsis

This paper describes a computer~program for estimating the amount of unbound or active drug from plasma values for cases in which there is multiple binding of a drug to a macromolecule such as serum albumin. The program is based upon the assumption that two sets of sites on the protein are involved in the binding and they have different binding affinities and capacities. The main input data consist of association constants and binding capacities which have been determined in separate studies such as equilibrium dialysis experiments. The output provides tables of estimates for values of bound and unbound drug in mg/1,/zmole/1, as well as per cent values at various plasma levels. The binding of dicumarol by human serum albumin is used as an illustrative example.

Drug-protein binding

Anticoagulant binding Unbound drug Active drug Multiple binding