A method for assessing dihydrofolate reductase inhibition in vivo
β Scribed by L.C. Mishra; A.S. Parmar; J.A.R. Mead
- Book ID
- 107711291
- Publisher
- Elsevier Science
- Year
- 1972
- Tongue
- English
- Weight
- 489 KB
- Volume
- 48
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
The ability of the enzyme dihydrofolate reductase to catalyze the formation of tetrahydrobiopterin from dihydrobiopterin was used to develop a method for measuring the activity ofthis enzyme in viva. This method can be used to determine the activity of the enzyme in tissues as well as the extent and
A flow microcalorimetric method was developed for the analysis of enzymatic activities in crude tissue homogenates. It can be applied whenever a heat exchange is involved in an enzymatic reaction. The consequent sensitivity obviously depends on the enthalpy variation observed. Dihydrofolate reductas