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A mass spectrometric procedure for rapid identification of trace volatile metabolites

✍ Scribed by Donald J. Parker; W.S. Ruliffson

Publisher
Elsevier Science
Year
1967
Tongue
English
Weight
423 KB
Volume
19
Category
Article
ISSN
0003-2697

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✦ Synopsis

Detection of trace amounts (lO-' to lo-l2 mole) of volatile metabolites evolved by actively respiring animal tissue preparations is being investigated in this laboratory. Ethylene has been reported to evolve from normal rats by Kokonov (1)) and from isolated rat liver mitochondria and rat intestinal mucosa by Ram Chandra and Spencer (2). One of the methods of ethylene detection requires trapping in Hg(ClO&, regeneration by LiCl addition, and detection by gas chromatography (3, 4). Although that method is specific and sensitive for ethylene, other components, which may be present in the incubation flask atmosphere, escape detection. Described here, is a method whereby the respiratory atmosphere contained in an isolated incubation flask is examined directly for volatile compounds by means of the mass spectrometer.

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