A mass spectrometric investigation of the reaction between 4,4′-vinylenedipyridine bis[2,2′ : 6′,2″-terpyridine platinum (II)] and the self-complementary oligonucleotide d(CpGpTpApCpG)
✍ Scribed by Gordon Lowe; James A McCloskey; Jinsong Ni; Tirayut Vilaivan
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 665 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0968-0896
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✦ Synopsis
4,4'-Vinylenedipyridine bis[2,2':6',2"-terpyridine platinum(II)], a potential intermolecular bis-intercalator of DNA, reacts slowly at PtII, the linker being displaced by nucleobases. Crystallization of 4,4'-vinylenedipyridine bis[2,2':6',2"-terpyridine platinum(II)] in the presence of the self-complementary oligonucleotide d(CpGpTpApCpG) gave a product which was analyzed by electrospray ionization mass spectrometry. Molecular mass measurements demonstrated ligation of one terpyridine PtII, which was shown by liquid chromatography-mass spectrometry to reside exclusively on deoxyguanosine. The terpyridine PtII was shown by tandem mass spectrometry to be attached to the 3'-terminal deoxyguanosine, consistent with N-7 substitution.