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A manual method of sequential edman degradation followed by dansylation for the determination of protein sequences

✍ Scribed by Maire E. Percy; Barbara M. Buchwald


Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
553 KB
Volume
45
Category
Article
ISSN
0003-2697

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A method is described for the elucidation of the peptide substrate phosphorylation specificity of a protein kinase. Peptide libraries with two to six degenerate positions and a length of seven or nine amino acids were generated directly on Sepharose beads by solidphase peptide synthesis according to