A low-cost thermoelectrically cooled tissue clamp for in vitro cyclic loading and load-to-failure testing of muscles and tendons
✍ Scribed by Marc-Olivier Kiss; Nicola Hagemeister; Annie Levasseur; Julio Fernandes; Bertrand Lussier; Yvan Petit
- Publisher
- Elsevier Science
- Year
- 2009
- Tongue
- English
- Weight
- 379 KB
- Volume
- 31
- Category
- Article
- ISSN
- 1350-4533
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✦ Synopsis
In vitro cyclic loading and load-to-failure testing of muscles and tendons require a reliable linking device between tissues and the actuator that can transmit high loads without slippage or tissue damage. This article describes a simple custom-made thermoelectrically cooled freeze clamp. The effectiveness of the clamp to transmit loads without tissue slippage was evaluated on 10 canine quadriceps femoris myo-tendinous junctions in both load-to-failure and cyclic loading settings. Dynamic cyclic loading during an extensive period of time was successfully achieved. Loads up to 4.84 kN were applied in quasi-static conditions without evidence of clamp slippage or failure.