A liquid culture method for rescuing immature embryos of eastern cottonwood

✍ Scribed by Michael A. Savka; Jeffrey O. Dawson; Jalmer J. Jokela; Robert M. Skirvin

• Publisher: Springer
• Year: 1987
• Tongue: English
• Weight: 549 KB
• Volume: 10
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00037306

✦ Synopsis

A one-step method to rescue immature embryos of eastern cottonwood (Populus deltoides Bartr.) is described. Plantlets developed from 83% of 25-day-old embryos grown in shaken culture on Murashige and Skoog (MS) liquid medium with 2.2 #m indole-3-acetic acid (IAA) and from 86% of embryos not supplemented with IAA. In contrast, when the MS medium was solidified with 0.8% agar, plantlets developed from 25% of 25-day-old embryos cultured on medium supplemented with IAA and from 28% of embryos in medium not supplemented with IAA. Eighty eight percent of all plantlets survived a gradual acclimitization to peat plugs in a greenhouse. The one-step liquid-culture method is an effective means of rescuing immature embryos by ovule culture from excised artificially-pollinated female branches in our cottonwood breeding program.