A liposome-based assay for quantitation of detergents
✍ Scribed by H⇘kan Eriksson; Bo Mattiasson
- Book ID
- 112896302
- Publisher
- Springer-Verlag
- Year
- 1982
- Tongue
- English
- Weight
- 173 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0273-2289
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📜 SIMILAR VOLUMES
Protein hydrophobicity is often detected by binding of protein to micelles of a mild detergent. A new fluorescence method for detection of this binding is presented. The method is based on a long-range quenching of tryptophan fluorescence by energy transfer to a pyrene-labeled phospholipid probe inc
Routine direct assessment of immunoglobulin (Ig)liposome(lp) conjugation efficiency has been impeded by phospholipid interference with standard protein and immunoassay methods. Rabbit IgG conjugated to anionic liposomes was quantitated in immunoblots using computer image analysis techniques. Lp-coup