A limitation of the continuous spectrophotometric assay for the measurement of myo-inositol-1-phosphate synthase activity
β Scribed by Xinyi Huang; Marcy Hernick
- Book ID
- 113412132
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 364 KB
- Volume
- 417
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A fluorescent water-soluble substrate for phosphatidylinositol-specific phospholipase C was synthesized. The diacylglycerol moiety of the natural substrate, phosphatidylinositol, was replaced by the fluorescent moiety, 2-naphthol, resulting in the synthetic substrate, racemic 2-naphthyl myo-inositol
We report a novel fluorometric end-point assay for the determination of 1-deoxy-D-xylulose 5-phosphate synthase (DXS) activity based on the reaction of 1-deoxy-D-xylulose 5-phosphate (DX5P) with 3,5-diaminobenzoic acid in an acidic medium to form a highly fluorescent quinaldine derivative. The assay