A leukaemic cell mutant with a thermolabile alanyl-transfer RNA synthetase
โ Scribed by SATO, KOKI
- Book ID
- 109697081
- Publisher
- Nature Publishing Group
- Year
- 1975
- Tongue
- English
- Weight
- 327 KB
- Volume
- 257
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/257813a0
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๐ SIMILAR VOLUMES
A continuous spectrophotometric assay is described for the aminoacylation reaction catalyzed by Escherichia coli alanyl-transfer RNA synthetase. The assay is based on coupling the alanyl-tRNA synthetase-dependent formation of AMP to the lactate dehydrogenase oxidation of NADH. Oxidation of NADH, as
A new continuous spectrophotometric assay is demonstrated for Escherichia coli alanyl-tRNA synthetase. It involves beta-gamma adenylyl imidophosphate as a substitute for ATP in the pyrophosphate exchange reaction. The net conversion of beta-gamma adenylyl imidophosphate to ATP can be linked to NADP