𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A lentiviral vector pseudotype suitable for vaccine development

✍ Scribed by Luciene Lopes; Marie Dewannieux; Yasuhiro Takeuchi; Mary K. Collins

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
217 KB
Volume
13
Category
Article
ISSN
1099-498X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Background

Lentiviral vectors (LV) are promising vaccines because they transduce dendritic cells (DC) in vivo. To translate LV vaccines into clinical trials, bulk production will be necessary. The present study aimed to find a suitable envelope for LV vaccine production from stable packaging cells because the commonly used vesicular stomatitis virus envelope (VSV‐G) is cytotoxic.

Methods

The envelope from Ross river virus (RRV) was selected. It can infect mouse and human cells, allowing testing in animals before clinical translation. We used VSV‐G for comparison. Vectors produced with each envelope were titred on human 293T cells and mouse 3T3 cells.

Results

RRV‐pseudotyped LV (RRV‐LV) infected mouse myeloid DC in culture and immunized mice. An approximately 50‐fold higher dose of RRV‐LV than VSV‐G‐LV was required to generate a similar T cell response. The RRV‐LV could also be used to infect human mDC and to prime a human T cell immune response.

Conclusions

RRV envelope is a suitable candidate to be used for the construction of an LV producer cell line. LV vaccines with RRV envelope can be tested in mice and in human immune cell cultures. The higher dose of RRV‐LV required for vaccine efficacy compared to VSV‐G‐LV will partly be offset by ease of production. Copyright © 2011 John Wiley & Sons, Ltd.

📜 SIMILAR VOLUMES

A lentiviral vector-based adenovirus fib
A lentiviral vector-based adenovirus fiber-pseudotyping approach for expedited functional assessment of candidate retargeted fibers
✍ Taco G. Uil; Jeroen de Vrij; Jort Vellinga; Martijn J. W. E. Rabelink; Steve J. 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 386 KB

## Abstract ## Background Many studies aimed at retargeting adenovirus (Ad) rationally focus on genetic modification of fiber, which is the primary receptor‐binding protein of Ad. Retargeted fibers ultimately require functional validation in the viral context. ## Methods Lentiviral vectors (LV)

Diverse genomic integration of a lentivi
Diverse genomic integration of a lentiviral vector developed for the treatment of Wiskott–Aldrich syndrome
✍ Julie Mantovani; Sabine Charrier; Ralph Eckenberg; William Saurin; Olivier Danos 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 323 KB

## Abstract ## Background The genomic integration of a lentiviral vector developed for the treatment of Wiskott–Aldrich syndrome (WAS) was assessed by localizing the vector insertion sites (IS) in a murine model of gene therapy for the disease. ## Methods Transduced hematopoietic progenitor cell

Development and characterization of a mi
Development and characterization of a minimal inducible packaging cell line for simian immunodeficiency virus-based lentiviral vectors
✍ Seraphin Kuate; Ralf Wagner; Klaus Überla 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 250 KB

## Abstract ## Background Lentiviral vectors allow gene transfer into non‐dividing cells. Further development of these vector systems requires stable packaging cell lines that enable adequate safety testing. ## Methods To generate a packaging cell line for vectors based on simian immunodeficienc