A laser Raman study of lysozyme denaturation
β Scribed by Randolph S. Porubcan; Kenneth L. Watters; James T. McFarland
- Book ID
- 115703862
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 715 KB
- Volume
- 186
- Category
- Article
- ISSN
- 0003-9861
No coin nor oath required. For personal study only.
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A multidisciplinary study of the thermal denaturation of lysozyme in the presence of three sulfoxides with different length in hydrocarbon chain (DMSO, DESO, and DPSO) was carried out by means of DSC, Raman spectroscopy, and SDS-PAGE techniques. In particular, the T d and DH values obtained from the
## Abstract Raman spectra of the pH denaturation of tropomyosin are presented. In the native state tropomyosin has an alphaβhelical content of nearly 90%, but this value drops rapidly as the pH is raised above 9.5. The Raman spectrum of the native state is characterized by a strong amide I line app