A large scale preparative gel electrophoresis separation of α1 and α2 subunits of the voltage-gated Ca2+ channel from rabbit skeletal muscle
✍ Scribed by Dr. Okot Nyormoi; Toni Schneider; Robert G. Smith
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 535 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0173-0835
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✦ Synopsis
A large scale preparative gel electrophoresis separation of a, and a2 subunits of the voltage-gated Ca2' channel from rabbit skeletal muscle A large-scale preparative gel electrophoresis method effectively separates individual voltage-gated calcium channel (VGCC) subunits with high resolution, starting with up to 4 mg of rabbit skeletal muscle L-type VGCC complex. Using this method, we separated a, and a, subunits of rabbit skeletal muscle VGCC with a high efficiency and with protein recoveries of 83%. The separated a, and a, subunits eluted from the gel in a 1:l molar ratio. The method should be applicable for separating the other VGCC subunits or subunits of other protein complexes.