A large, efficient and inexpensive bacterial growth chamber

✍ Scribed by Vern L. Schramm

• Book ID: 102629834
• Publisher: Elsevier Science
• Year: 1974
• Tongue: English
• Weight: 314 KB
• Volume: 57
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(74)90092-x

✦ Synopsis

In the preparation of a~lenosinc~ monol~h0spllatc~ nucleosiflasc [ mp nuclcoeiclnse, (EC 3.2.2.4 I 1 from Azotobacter ~linelnndii c)P (1,2 I and Ewhf,).icl2iu coli K,,, it n-:~s ncccwu-p to use kilogram quantities of cell m:ts~ :LS starting material. In order to proridc these without using continuous culture teclmiqucti, :I 200 liter batch-type growth chnmher xvas const,ructcd from relatively inexpensive and readily availnhle mntcrials. Tl?c cahnml)er was designed to pwvirlr high rates of nuxtion since both organisms were grown nerohicnlly. The growth cbhar:wtrrist,irs of both A. I'irlelnndii and E. coli in the growth rhamher are described and compared to the growth ch:~rncteristics of the organisms in 2 liter shaken fhks.