Front Matter....Pages I-XIV
An Introduction to Avidin-Biotin Technology and Options for Biotinylation....Pages 1-29
Synthesis of Photocleavable Biotinylated Ligands and Application for Affinity Chromatography....Pages 31-44
Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands....Pages 45-63
Photoreactive Biotinylated Peptide Ligands for Affinity Labeling....Pages 65-81
Immunoprecipitation of Biotinylated Cell Surface Proteins....Pages 83-97
Biotinylation and Chemical Cross-Linking of Membrane Associated Molecules....Pages 99-114
Preparation of Biotinylated Lectins and Application in Microtiter Plate Assays and Western Blotting....Pages 115-129
Biotin-Labeling of Poly(ADP-ribose) in Poly(ADP-ribose)-Protein Interactions....Pages 131-141
Preparation and Use of Biotinylated Probes for the Detection and Characterisation of Serine Proteinase and Serine Proteinase Inhibitory Proteins....Pages 143-165
Avidin/Biotin-Mediated Conjugation of Antibodies to Erythrocytes: An Approach for in vivo Immunoerythrocyte Exploration....Pages 167-182
Biotin in vitro Translation: A Nonradioactive Method for the Synthesis of Biotin Labeled Proteins in a Cell-Free System....Pages 183-199
Nonradioactive Detection of Nucleic Acids with Biotinylated Probes....Pages 201-213
Biotin-Labeled Riboprobes to Study RNA-Binding Proteins....Pages 215-225
Rapid YAC End Sequencing by Alu-Vector PCR and Biotinylated Primers....Pages 227-237
Back Matter....Pages 238-240