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A laboratory exercise in comparative DNA analysis

โœ Scribed by Lesley R. Brown

Publisher
The American Society for Biochemistry and Molecular Biology
Year
2003
Tongue
English
Weight
47 KB
Volume
31
Category
Article
ISSN
1470-8175

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โœฆ Synopsis

Abstract

This report describes a laboratory exercise that is used in an upper level biochemistry course at a small liberal arts college. The laboratory exercise was designed with three major learning objectives in mind. The first objective is that students learn how to isolate and purify plasmid DNA. The second is that they understand this process on a molecular level. Last, they analyze their DNA samples using a variety of biochemical and biophysical methods. By comparing and contrasting the results, they are to draw conclusions about which methods would be best under a given set of experimental conditions.

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Cytologic evaluation of body cavityfluids is useful to detect malignancy within the pleural and peritoneal spaces. A definitive diagnosiv cannot always be made on cytologic evaluation alone. As malignant processes may show abnormal DNA content, DNA analysis of effusions may be useful. Therefore, we