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A J-Multiplied HMQC (MJ-HMQC) Experiment for Measuring 3JHNHα Coupling Constants

✍ Scribed by Youlin Xia; Xiangming Kong; Nancy Ip; Guang Zhu

Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
68 KB
Volume
146
Category
Article
ISSN
1090-7807

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✦ Synopsis

amplifies J coupling constants m times and allows direct observation of the 3 J HNH␣ coupling constants of peptides and proteins (<10 kDa). The drawbacks to this method are line broadening in the f 1 -dimension and lower sensitivity. In the J-multiplied HMQC (MJ-HMQC) experiment described here, the PEP-HSQC pulse sequence is replaced by a sensitivity-enhanced HMQC section, and the total decay time for the J-coupling and the chemical shift evolution is shortened by a period of t 1 . This experiment affords narrower linewidth and enhances the sensitivity by 34%, on an average of 105 well-isolated peaks, when compared with the MJ-HSQC experiment.

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