A hybrid sigma subunit directs RNA polymerase to a hybrid promoter in Escherichia coli
โ Scribed by Ashok Kumar; Brenda Grimes; Mary Logan; Stephen Wedgwood; Helen Williamson; Richard S. Hayward
- Book ID
- 117143898
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 967 KB
- Volume
- 246
- Category
- Article
- ISSN
- 0022-2836
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๐ SIMILAR VOLUMES
A mutant of Escherichia coli K12 is described in which sigma and alpha subunits of the DNA-dependent RNA polymerase (EC 2.7.7.6) are produced at the rates much higher than in the normal strain. The rate of synthesis for sigma subunit was found to be at least 10-times higher, though the rapid degrada
A cold-sensitive mutation in the rpoB gene for the RNA polymerase beta subunit increasing the temperature of promoter opening on T2 phage DNA was obtained in Escherichia coli. The mutation also affects the stages preceding promoter opening by increasing the dissociation rate of RNA polymerase--DNA c