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A Homogeneous G Protein-Coupled Receptor Ligand Binding Assay Based on Time-Resolved Fluorescence Resonance Energy Transfer

✍ Scribed by Hu, Liaoyuan A.; Zhou, Tian; Hamman, Brian D.; Liu, Qingyun


Book ID
115513471
Publisher
Mary Ann Liebert
Year
2008
Tongue
English
Weight
721 KB
Volume
6
Category
Article
ISSN
1540-658X

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An assay named Cell TR-FRET based on time-resolved fluorescence resonance energy transfer, here utilized for detection of receptor proteins on intact cells, is described. In this assay, intact membrane-biotinylated Sf9 cells expressing human interleukin-2Ralpha due to infection with a recombinant ba