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A histochemical study about the influence of lytic enzymes on plasma membrane enzyme activities in rat liver and kidney

✍ Scribed by M. J. Hardonk; T. J. Meskendorp-Haarsma; J. Koudstaal

Publisher
Springer
Year
1978
Tongue
English
Weight
264 KB
Volume
58
Category
Article
ISSN
1432-119X

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✦ Synopsis

  1. The effect of lipolytic, glycolytic and proteolytic enzymes on the activities of plasma membrane enzyme activities in rat liver and kidney has been investigated by a pretreatment of tissue sections with the lytic enzymes. 2. The action of the proteolytic enzymes causes a very strong decrease of leucyl-beta-naphthylamidase activity, whereas the activities of ATP-ase, 5'-nucleotidase and alkaline phosphatase show a lesser decrease. This indicates a different membrane anchorage of leucyl-beta-naphthylamidase as compared to that of the phosphatases. 3. Treatment with glycolytic enzymes results in a decrease of 5'-nucleotidase and ATP-ase activity, whereas liver alkaline phosphatase and leucyl-beta-naphthylamidase show an increase in activity. 4. Treatment with phospholipase C gives about the same results. The very strong decrease of 5'-nucleotidase activity indicates a great dependence on phospholipids.

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