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A histochemical method for β-hydroxyacyl CoA

✍ Scribed by I. Diculesco; D. Onicesco

Book ID
104852141
Publisher
Springer
Year
1965
Tongue
English
Weight
297 KB
Volume
52
Category
Article
ISSN
0028-1042

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Enzymatic assay for 3-hydroxyacyl-CoA an
Enzymatic assay for 3-hydroxyacyl-CoA and 2-trans-enoyl-CoA intermediates of β-oxidation
✍ Pirjo M. Latipää; Ilmo E. Hassinen; J. Kalervo Hiltunen 📂 Article 📅 1988 🏛 Elsevier Science 🌐 English ⚖ 489 KB

An enzymatic assay is presented for determining 3-hydroxyacyl-CoA and 2-trans-enoyl-CoA esters in tissue samples. The procedure includes extraction of acyl-CoA esters from frozen tissue samples with chloroform/methanol, stochiometric oxidation of the acyl esters to 3-keto-acyl-CoAs with 3-hydroxyacy