A highly sensitive peroxidase-antiperoxidase method for detection of H-Y antigen on cultivated human fibroblasts

✍ Scribed by U. Müller; K. Bross

• Publisher: Springer
• Year: 1979
• Tongue: English
• Weight: 275 KB
• Volume: 52
• Category: Article
• ISSN: 0340-6717
• DOI: 10.1007/bf00284609

✦ Synopsis

A peroxidase-anti-perioxidase method for the detection of H-Y antigen at the single cell level is described. The efficiency of the test was examined in cultivated fibroblasts derived from control subjects and from XX males and a true hermaphrodite. For comparison, H-Y antigen was determined in blood cells of the same probands using the cytotoxic test. The finding of H-Y positive fibroblasts in the intersex patients has implications for the origin of these disorders.