We evaluated the diagnostic value of Flinders Technology Associates (FTA) filter paper together with polymerase chain reaction (PCR) for detection of Pneumocystis jirovecii (carinii) from induced sputum (IS) and bronchoalveolar lavage fluid (BALF) samples. The study involved 162 patients with clinic
✦ LIBER ✦
A highly sensitive novel PCR assay for detection of Pneumocystis jirovecii DNA in bronchoalveloar lavage specimens from immunocompromised patients
✍ Scribed by T. Tia; C. Putaporntip; R. Kosuwin; N. Kongpolprom; K. Kawkitinarong; S. Jongwutiwes
- Book ID
- 115245870
- Publisher
- John Wiley and Sons
- Year
- 2012
- Tongue
- English
- Weight
- 165 KB
- Volume
- 18
- Category
- Article
- ISSN
- 1198-743X
No coin nor oath required. For personal study only.
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Quantitation of cytomegalovirus DNA in c
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## Abstract A novel nested quantitative‐competitive polymerase chain reaction (nQC‐PCR) assay was developed to quantify as few as ten copies per tube of human cytomegalovirus DNA with an overall dynamic range of 10–10^5^ copies per tube. This nQC‐PCR assay is based on co‐amplification of a mimic DN