## Abstract **Fast detection** of cellular thiols in aqueous medium was achieved using a newly developed fluorescence probe (see picture). Based on this probe, a high‐throughput fluorescence assay for glutathione reductase was developed.magnified image
✦ LIBER ✦
A Highly Sensitive Fluorescence Probe for Fast Thiol-Quantification Assay of Glutathione Reductase
✍ Scribed by Long Yi; Heyang Li; Lu Sun; Liangliang Liu; Caihong Zhang; Zhen Xi
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 421 KB
- Volume
- 121
- Category
- Article
- ISSN
- 0044-8249
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## Abstract A highly sensitive fluorogenic probe for tiopronin was proposed. 2,4‐Dinitrobenzenesulfonyl‐fluorescein (I) is an almost nonfluorescent compound. Upon mixing with tiopronin in aqueous solution, the 2,4‐dinitrobenzenesulfonyl group of I was efficiently removed and its parent dye fluoresc