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A highly sensitive assay for S-adenosylmethionine by high-performance liquid chromatography

✍ Scribed by William J. Burke

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
272 KB
Volume
122
Category
Article
ISSN
0003-2697

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✦ Synopsis

A highly sensitive assay for S-adenosylmethionine (AdoMet) by high-performance liquid chromatography with amperometric detection is described. The method is based on the measurement of 3-methoxytyramine formed from AdoMet and dopamine in the presence of catechol-O-methyltransferase. Using this technique 1 pmol of AdoMet can be measured and the AdoMet content of less than 1 mg of adrenal tissue can be accurately determined. This method is particularly suitable for assay of AdoMet in small amounts of tissues obtained by microsampling techniques.

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