A highly efficient and versatile microchip capillary electrophoresis method for DNA separation using gold nanoparticle as a tag

✍ Scribed by Wei Cao; Lei Chen; Youjian Fu; Zhenyi Tan; Bin Qu

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 269 KB
• Volume: 34
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.201000755

✦ Synopsis

Abstract

A highly efficient and versatile method for DNA separation using Au nanoparticles (Au NPs) as a tag based on microchip capillary electrophoresis (MCE) was developed. The thiol‐modified DNA‐binding Au NPs were utilized as a tag. Target DNA was sandwiched between Au NPs and probe DNA labeled with horseradish peroxidase (HRP). In electrophoresis separation, the difference in electrophoretic mobility between free probe and probe‐target complex was magnified by Au NPs, which enabled the resulting mixture to be separated with high efficiency by microchip capillary electrophoresis. Horseradish peroxidase was used as a catalytic label to achieve sensitive electrochemical DNA detection via fast catalytic reactions. With this protocol, 27‐mer DNA fragments with different sequences were separated with high speed and high resolution. The proposed method was critical to achieve improved DNA separations in hybridization analyses.