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A highly diastereoselective vinylogous Mannich condensation and 1,4-conjugate addition of (Z)-propenyl cuprate in the synthesis of an influenza neuraminidase inhibitor

✍ Scribed by David M. Barnes; Lakshmi Bhagavatula; John DeMattei; Ashok Gupta; David R. Hill; Sukumar Manna; Maureen A. McLaughlin; Paul Nichols; Ramiya Premchandran; Michael W. Rasmussen; Zhenping Tian; Steven J. Wittenberger


Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
346 KB
Volume
14
Category
Article
ISSN
0957-4166

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✦ Synopsis


A practical synthesis of neuraminidase influenza inhibitor, A-322278, has been developed. Asymmetry is introduced into the synthesis by an enzyme mediated ester hydrolysis. A highly diastereoselective vinylogous Mannich condensation reaction of N-Boc-2-tert-butyldimethylsilyloxypyrrole (TBSOP) and an N-(triphenylmethylsulfenyl)imine proceeds under thermodynamic control to assemble the framework. A significant temperature dependent rate difference for the transfer of (Z)-and (E)-propenyl moieties from a cuprate reagent during a 1,4-conjugate addition was observed. A very selective addition of cyanide to an N-acyliminium intermediate was employed to control the final stereocenter.