✦ LIBER ✦

A High-Throughput Assay for Mitochondrial Membrane Potential in Permeabilized Yeast Cells

✍ Scribed by Ellyn Farrelly; M.Catherine Amaral; Lisa Marshall; Shu-Gui Huang

Book ID: 102559391
Publisher: Elsevier Science
Year: 2001
Tongue: English
Weight: 112 KB
Volume: 293
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.2001.5139

No coin nor oath required. For personal study only.

✦ Synopsis

A fluorometric assay for mitochondrial membrane potential in permeabilized yeast cells has been developed. This method involves permeabilizing the plasma membrane and measuring the distribution of a mitochondrial membrane potential sensitive probe 3,3dipropylthiadicarbocyanine iodide (DiSC 3 (5); DiSC 3 ). In permeabilized cells, DiSC 3 fluorescence decreased when introduced into energized mitochondria and increased three-to sixfold when the mitochondrial membrane potential was dissipated by the chemical uncoupler carbonylcyanide m-chlorophenyl hydrazone. Plasma membrane potential was abolished by permeabilization, as shown by a lack of polarization of the plasma membrane induced by K ؉ and glucose. Uncoupling protein 1 (UCP1), a mitochondrial H ؉ transporter, was used as a model for method validation. The fluorescence intensity responded vigorously to specific modulators in UCP1-expressing cells. This method has been adapted as a high-throughput assay to screen for modulators of mitochondrial membrane potential.

📜 SIMILAR VOLUMES

Biomimetic macroporous hydrogel scaffold

Biomimetic macroporous hydrogel scaffolds in a high-throughput screening format for cell-based assays

✍ Maria B. Dainiak; Irina N. Savina; Isabella Musolino; Ashok Kumar; Bo Mattiasson 📂 Article 📅 2008 🏛 American Institute of Chemical Engineers 🌐 English ⚖ 523 KB

Development of a high-throughput membran

Development of a high-throughput membrane-array method for molecular diagnosis of circulating tumor cells in patients with gastric cancers

✍ Chan-Hang Wu; Shiu-Ru Lin; Fang-Jung Yu; Deng-Chyang Wu; Yong-Sang Pan; Jan-Sing 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 French ⚖ 374 KB 👁 1 views

## Abstract Recently several noninvasive methods have been employed to detect circulating tumor cells (CTCs) in cancer patients. In this study, we have developed a highly sensitive, high‐throughput colorimetric membrane‐array method that was designed to detect a panel of mRNA markers including huma

Detection of a decrease in green fluores

Detection of a decrease in green fluorescent protein fluorescence for the monitoring of cell death: An assay amenable to high-throughput screening technologies

✍ Ann-Muriel Steff; Marylène Fortin; Chantal Arguin; Patrice Hugo 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 223 KB 👁 3 views

Change in Mitochondrial Membrane Potenti

Change in Mitochondrial Membrane Potential in Peripheral Blood Lymphocytes, Especially in Natural Killer Cells, Is a Possible Marker for Surgical Stress on the Immune System

✍ Arimichi Takabayashi; Michiyuki Kanai; Yasuhiro Kawai; Shingo Iwata; Tetsuro Sas 📂 Article 📅 2003 🏛 Springer 🌐 English ⚖ 178 KB

A Reporter Gene Assay for High-Throughpu

A Reporter Gene Assay for High-Throughput Screening of G-Protein-Coupled Receptors Stably or Transiently Expressed in HEK293 EBNA Cells Grown in Suspension Culture

✍ Yves Durocher; Sylvie Perret; Eric Thibaudeau; Marie-Helene Gaumond; Amine Kamen 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 184 KB

We describe in detail a robust, sensitive, and versatile functional assay for G-protein-coupled receptors (GPCRs) expressed in human embryonic kidney (HEK) 293-EBNA (Epstein-Barr virus nuclear antigen) (designated 293E) cells. The ability to grow these cells in suspension, in conjunction with the us