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A high phosphotyrosine phosphatase activity is present in differentiating bovine lens cells

✍ Scribed by Pierre R. Blanquet; Frederic Croquet


Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
333 KB
Volume
19
Category
Article
ISSN
1065-6995

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✦ Synopsis


Activity of phosphotyrosine ‐ protein phosphatases (PTPases) has been investigated in the different cellular regions of bovine eye lens. PTPases were tested in cellular detergent extracts using phospholabelled synthetic peptides and p‐nitrophenyl phosphate. We show that a high PTPase activity is only present in cells which undergo differentiation, namely the equatorial epithelium and cortex fiber cells. Since this activity is found to be severely inhibited by a specific inhibitor of receptor ‐ type PTPases, it can be suggested that one or more members of this class of PTPases might play an important role in the lens differentiation process.