✦ LIBER ✦
A high-performance liquid chromatographic technique that separates cellular retinol binding protein from cellular retinoic acid binding protein
✍ Scribed by Fabrizio C. Bonelli; Luigi M. De Luca
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 501 KB
- Volume
- 147
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
A one-step procedure to detect cellular [3H]retinol and ['Hlretinoic acid binding proteins (CRBP and CRABP) from rat testis cytosolic extract was devised. The procedure is based on anion-exchange high-performance liquid chromatography of the cytosolic fraction on columns of Mono Q, which permits elution of CRABP and CRBP at 12 and 22 min, respectively.