𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A high amount of satellite DNA in the genome ofLupinus angustifoliusL.

✍ Scribed by Katrien Strubbe; Patrick Oostveldt; Daniel Broekaert; Roger Parijs

Publisher
Springer-Verlag
Year
1982
Tongue
English
Weight
516 KB
Volume
155
Category
Article
ISSN
0032-0935

No coin nor oath required. For personal study only.

✦ Synopsis

Embryo DNA, isolated from ungerminated seeds of Lupinus angustifolius L., contains an exceptionally high amount of guanine-cytosine-rich satellite DNA. The thermal denaturation curve of total embryo DNA is biphasic with an inflexion point at 62% denaturation, indicating the presence of satellite DNA. The satellite fraction could be separated from the mainband DNA by three successive preparative CsCl-gradient centrifugations. The densities of the DNA fractions are 1.7045 g cm -3 and 1.6925 g cm -3, respectively. The percentages of guanine-cytosine calculated from these densities are comparable to the percentages of GC calculated from the melting temperatures. Finally, reassociation studies prove that foldback DNA and highly repeated sequences are much more frequent in the satellite DNA fraction than in the mainband DNA.

πŸ“œ SIMILAR VOLUMES

The incidence of mini- and micro-satelli
The incidence of mini- and micro-satellite repetitive DNA in the canine genome
✍ J. Rothuizen; J. Wolfswinkel; J. A. Lenstra; R. R. Frants πŸ“‚ Article πŸ“… 1994 πŸ› Springer 🌐 English βš– 524 KB

We have estimated the incidence of microand mini-satellites in the dog genome. A genomic phage library from canine liver, with an average insert size of 16 kb, was screened to detect potentially polymorphic microand mini-satellite sequences, which may be useful for the development of markers of inhe