A hand-held photometer for quantitative dot-immunobinding assays

✍ Scribed by Clement Bordier; Philippe Ryter

• Book ID: 102984974
• Publisher: Elsevier Science
• Year: 1986
• Tongue: English
• Weight: 881 KB
• Volume: 152
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(86)90128-4

✦ Synopsis

A simple multichannel transmission photometer is presented. This instrument is designed to give a quantitative result from dot-immunobinding assays. The optoelectronic components of the system (yellow GaP light-emitting diode and CdS photoresistor) have been selected to match the absorbance maximum of the product generated by the peroxidase-catalyzed oxidation of 4chloro-I-naphthol. The standard deviation of duplicate measures is lower than 0.5% on a single channel and lower than I .5% on different channels. The photometer is able to read linearly the absorbance of samples up to a value of A 5sr = 3.0. As an application example, the photometer was used to read the results of the titration of sera of Leishmania-infected dogs on Leishmania donovani antigeIIS.