An Automated Aequorin Luminescence-Based
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Mark D. Ungrin; Laila M.R. Singh; Rino Stocco; Dean E. Sas; Mark Abramovitz
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Article
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1999
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Elsevier Science
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English
⚖ 145 KB
We describe in detail an automated and highly sensitive functional assay for calcium-coupled receptors (those receptors whose activation results in an increase in intracellular calcium levels) utilizing coelenterazine-charged aequorin as a probe for intracellular calcium levels ([Ca 2؉ ] i ). The as