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A GeNorm algorithm-based selection of reference genes for quantitative real-time PCR in skin biopsies of healthy dogs and dogs with atopic dermatitis

✍ Scribed by Yvette M. Schlotter; Eveline Z. Veenhof; Bas Brinkhof; Victor P.M.G. Rutten; Bart Spee; Ton Willemse; Louis C. Penning

Book ID: 116966478
Publisher: Elsevier Science
Year: 2009
Tongue: English
Weight: 272 KB
Volume: 129
Category: Article
ISSN: 0165-2427
DOI: 10.1016/j.vetimm.2008.12.004

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## Background Real-time quantitative PCR can be a very powerful and accurate technique to examine gene transcription patterns in different biological conditions. One of the critical steps in comparing transcription profiles is accurate normalisation. In most of the studies published on real-time PC