A bacterially expressed single chain antibody (scFv215) directed against the largest subunit of drosophila RNA polymerase II was analysed. Structure and function of the antigen binding site in scFv215 were probed by chain shuffling and by site-specific mutagenesis. The entire variable region of eith
✦ LIBER ✦
A Genetically Encoded Direct Sensor of Antibody–Antigen Interactions
✍ Scribed by Jeremy H. Mills; Hyun Soo Lee; Chang C. Liu; Jiangyun Wang; Peter G. Schultz
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 301 KB
- Volume
- 10
- Category
- Article
- ISSN
- 1439-4227
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## BACKGROUND. To develop a new immunologic approach to multiple myeloma, the authors generated a monoclonal antibody against a human plasma cell specific antigen, HM1.24. Their previous study showed the antitumor effect of this antibody in severe combined immunodeficiency (SCID) mice bearing human