A genetic study of various enzyme polymorphisms inPleurodeles waltlii(Urodele, Amphibian). III. The relationship between sex-linked peptidase-1 expression and gene-dose effects
✍ Scribed by Christian Cayrol; André Jaylet; Vincent Ferrier; François Gasser
- Book ID
- 104783967
- Publisher
- Springer
- Year
- 1983
- Tongue
- English
- Weight
- 416 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0006-2928
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✦ Synopsis
Erythrocyte peptidase-1 was analyzed by electrophoresis in various types of triploid Pleurodeles waltlii. Densitometric analysis of the zymograms showed (1) the existence of a gene-dose effect and (2) the presence of two Pep-1B alleles for one Pep-1A allele in heterozygous triploid females of biparental origin. Owing to the sex linkage of the PEP-1 locus (alleles Pep-1A and Pep-1B situated on the Z and W sex chromosomes, respectively), the results show that the sex genotype of these females is ZWW. In a particular line called series 103, the existence of a null allele was demonstrated. Densitometric analysis of females which were Pep-1A/Pep-10 (ZW), Pep-10/Pep-10 (WW), and Pep-1A/Pep-10/Pep-10 (ZWW) confirmed the gene-dose effect.