## Abstract Nitrate reductase was investigated in extracts from cells of a chlorateโresistant mutant strain of __E. coli__ which grew anaerobically on nitrate as the sole source of nitrogen. The nitrate reductase was of particulate nature and reduced chlorate like the nitrate reductase from the wil
A genetic study of apramycin-resistant mutants of Escherichia coli
โ Scribed by Dr. Branka Vasiljevic; Djordje Fira; Ljubisa Topisirovic
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 329 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0233-111X
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โฆ Synopsis
Abstract
The apramycinโresistant mutants of E. coli were isolated spontaneously. Mutations, aprA and aprB, conferring resistance to apramycin were located at 72 min on the E. coli genetic map where most of ribosomal genes were mapped. One of the mutants, carrying the aprB mutation, has an altered translational fidelity expressed as severe restriction of amber suppressor activity in vivo. The chromosome location and altered translational fidelity indicate that the apramycinโresistant phenotype could be a consequence of an alteration of the ribosomal structure.
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