A genetic linkage map ofPicea abiesKarst., based on RAPD markers, as a tool in population genetics

✍ Scribed by G. Binelli; G. Bucci

• Publisher: Springer
• Year: 1994
• Tongue: English
• Weight: 852 KB
• Volume: 88-88
• Category: Article
• ISSN: 0040-5752
• DOI: 10.1007/bf00223633

✦ Synopsis

Norway spruce (Picea abies Karst.) is a most important species among European forest trees for both economical and ecological reasons. However, this species has suffered from a lack of information on the genetic side due to the scarcity of linkage data. In this study we have used a population of 72 megagametophytes from a single tree in a natural Italian stand to produce a genetic linkage map by means of RAPD markers. Ninety-six random decamers used as primers yielded 185 polymorphic loci showing Mendelian inheritance. Analysis of the segregation by multipoint analysis allowed us to define 17 major linkage groups covering a total distance of 3584 cM, with an average spacing between markers of 22 cM. Possible uses of a genetic linkage map with respect to population ecology and genetics are discussed.