✦ LIBER ✦

A generic protocol for the purification and characterization of water-soluble complexes of affinity-tagged proteins and lipids

✍ Scribed by Maeda, Kenji; Poletto, Mattia; Chiapparino, Antonella; Gavin, Anne-Claude

Book ID: 126792798
Publisher: Nature Publishing Group
Year: 2014
Tongue: English
Weight: 814 KB
Volume: 9
Category: Article
ISSN: 1750-2799
DOI: 10.1038/nprot.2014.148

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Synthesis and characterization of a wate

Synthesis and characterization of a water-soluble affinity polymer for trypsin purification

✍ J. H. T. Luong; K. B. Male; A. L. Nguyen 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 539 KB

Current strategies for the use of affini

Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins

✍ José Arnau; Conni Lauritzen; Gitte E. Petersen; John Pedersen 📂 Article 📅 2006 🏛 Elsevier Science 🌐 English ⚖ 555 KB

The AviD-tag, a NeutrAvidin/avidin speci

The AviD-tag, a NeutrAvidin/avidin specific peptide affinity tag for the immobilization and purification of recombinant proteins

✍ Thomas Gaj; Scott C. Meyer; Indraneel Ghosh 📂 Article 📅 2007 🏛 Elsevier Science 🌐 English ⚖ 804 KB

Inexpensive and Generic Affinity Purific

Inexpensive and Generic Affinity Purification of Recombinant Proteins Using a Family 2a CBM Fusion Tag

✍ Beatriz Rodriguez; Mojgan Kavoosi; Jürgen Koska; A. Louise Creagh; Douglas G. Ki 📂 Article 📅 2004 🏛 American Institute of Chemical Engineers 🌐 English ⚖ 183 KB

Plasmid system for the intracellular pro

Plasmid system for the intracellular production and purification of affinity-tagged proteins in Bacillus megaterium

✍ Rebekka Biedendieck; Yang Yang; Wolf-Dieter Deckwer; Marco Malten; Dieter Jahn 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 489 KB 👁 1 views

## Abstract A multiple vector system for the intracellular high‐level production of affinity tagged recombinant proteins in __Bacillus megaterium__ was developed. The N‐ and C‐terminal fusion of a protein of interest to a Strep II and a His~6~‐tag is possible. Corresponding genes are expressed unde

Reprint of: Current strategies for the u

Reprint of: Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins

✍ Arnau, José; Lauritzen, Conni; Petersen, Gitte E.; Pedersen, John 📂 Article 📅 2011 🏛 Elsevier Science 🌐 English ⚖ 582 KB