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A generally applicable technique for the extraction of restrictable DNA from methanogenic bacteria

โœ Scribed by Stephanie A. Meakin; John H.E. Nash; William D. Murray; Kevin J. Kennedy; G.Dennis Sprott

Book ID
119126188
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
638 KB
Volume
14
Category
Article
ISSN
0167-7012

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โœ Awdhesh Kalia; Ashok Rattan; Prem Chopra ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 143 KB

In this study, we report a modified procedure for extraction of high-quality genomic DNA that is rapid, simple, biologically nonhazardous, and generally applicable to pathogenic bacteria. Bacterial cells were pretreated with 70% ethanol prior to enzymatic digestion with lysozyme. Exposure of bacteri