A general procedure for evaluation of immunological relevance of synthetic peptides: Peptides synthesized on paper in enzyme-linked immunosorbent assay
✍ Scribed by Viktor Krchňák; Josef Vágner; Jan Novák; Alena Suchánková; Jaroslav Roubal
- Book ID
- 102630426
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 428 KB
- Volume
- 189
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Multiple continuous-flow solid-phase peptide synthesis has been adapted for synthesis of peptides on a cellulose carrier (Whatman 3MM paper). Paper-bound synthetic peptides that represent antigenic determinants of particular proteins detected antibodies against the respective proteins in an enzyme-linked immunosorbent assay. The method is applied to the synthesis, and use in site-directed serology, of four peptides derived from the gp41 glycoprotein of HIV, the Epstein-Barr virus-determined nuclear antigen-1 and VCA proteins of the Epstein-Barr virus, and the early region of human papillomavirus type 11.