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A general NMR method for the assay of racemase activity with optically active or optically inactive substrates

✍ Scribed by U.M. Babu; R.B. Johnston; L.C. McNeff

Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
308 KB
Volume
63
Category
Article
ISSN
0003-2697

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✦ Synopsis

A rapid and sensitive method is described for determining the activity of alanine racemase (5. I. I. I) with its substrates. Alanine racemase from Bacillus subtilis catalyzes the exchange of the (Y hydrogen of L and D alanine, a racemic mixture of alanine as we!! as both the Q hydrogens of glycine with D,O. The rate of exchange which can be followed by NMR spectroscopy is proportional to the enzyme concentration. This assay has been used to test a wide variety of possible substrates and thus determine the specificity of the enzyme.

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