A general method for isolation of Mu d 1–8(Apr lac) operon fusions in Salmonella typhimurium LT2 from Tn10 insertion strains: chlC::Mu d 1–8

✍ Scribed by Kwan, H. S. ;Wong, K. K.

• Book ID: 104770885
• Publisher: Springer
• Year: 1986
• Tongue: English
• Weight: 358 KB
• Volume: 205
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00430431

✦ Synopsis

A general method was developed for the isolation of Salmonella thyphimurium LT2 Mu dl-8 (Aprlac) operon fusions in a gene displacing a TnlO insertion. Random Mu dl-8 fusion pools were prepared to grow phage P22 lysates which transduced chlC: :Tnl0 to AprTet s on fusaric acidampicillin plates. Among these Ap~Teff potential chiC: :Mu d18 fusions, a simple spot test identified the fusions that were closely linked to the TnlO insertion in chiC. Out of 68 Ap~Tet ~ isolates 7 chlC:: Mu dl-8 fusions with a nitrateinduced Lac + phenotype were obtained. When oxrA': TnlO was transduced into these ehlC::Mu dl-8 fusions, they became Lac-even in the presence of nitrate, confirming that they were chlC::Mu dl-8 fusions.