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A general method for determining the replicative age of normal animal cell cultures

โœ Scribed by James R. Smith; Olivia M. Pereira-Smith; Karen I. Braunschweiger; Thomas W. Roberts; Ronald G. Whitney


Book ID
119025110
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
610 KB
Volume
12
Category
Article
ISSN
0047-6374

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Glucose and lactate metabolic rates were evaluated for cultures of cord blood (CB) mononuclear cell (MNC), peripheral blood (PB) MNC, and PB CD34 + cell cultures carried out in spinner flasks and in T-flasks in both serum-containing and serum-free media. Specific glucose uptake rates (q gluc , in mi