𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A General Fluorogenic Assay for Catalysis Using Antibody Sensors

✍ Scribed by Paul Geymayer; Nicolaus Bahr; Jean-Louis Reymond

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
189 KB
Volume
5
Category
Article
ISSN
0947-6539

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Selective assay for heavy metal toxicity
Selective assay for heavy metal toxicity using a fluorogenic substrate
✍ Keumhee Jung; Gabriel Bitton; Ben Koopman 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 47 KB

Chromogenic substrates have been generally used in enzymatic assays for the specific determination of heavy metal toxicity. A toxicity assay based on the specific inhibition of ␤-galactosidase by heavy metals and using a fluorogenic substrate was evaluated for its sensitivity to heavy metals and org

A microtiter plate assay for N-acetyl-β-
A microtiter plate assay for N-acetyl-β-d-glucosaminidase using a fluorogenic substrate
✍ S. Linko-Löppönen; M. Mäkinen 📂 Article 📅 1985 🏛 Elsevier Science 🌐 English ⚖ 295 KB

We describe a simple endpoint method for the determination ofN-acetyl-@ghtcosaminidase (NAGase; EC 3.2.1.30). NAGase uses a fluorogenic substrate, 4-methylumbelliferyl-IV-acetyl-@tx-ghtcosaminide, at pH 4.6, liberating the fluorescent 4-methylumbelliferone. The method is reproducible and fast both a

Enzyme Kinetics Determined Using Calorim
Enzyme Kinetics Determined Using Calorimetry: A General Assay for Enzyme Activity?
✍ Matthew J. Todd; Javier Gomez 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 119 KB

Two techniques for determining enzyme kinetic constants using isothermal titration microcalorimetry are presented. The methods are based on the proportionality between the rate of a reaction and the thermal power (heat/time) generated. (i) An enzyme can be titrated with increasing amounts of substra

An assay for estradiol receptors using a
An assay for estradiol receptors using a staphylococcal protein-a — estradiol antibody adsorbent
✍ Enrico Ronchi; Carla Porta; Giovanni di Fronzo; Enrico Clerici 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 French ⚖ 748 KB

## Abstract Antiestradiol antisera were raised in rabbits by immunization with a steroid‐protein conjugate Whole antisera and purified antiestradiol antibodies were reacted with heat‐killed and formalin‐fixed bacteria from the protein‐A‐bearing strain of __Staphilococcus aureus__ (Cowan 1, NCTC 853