A general, fast, and sensitive micromethod for DNA determination: Application to rat and mouse liver, rat hepatoma, human leukocytes, chicken fibroblasts, and yeast cells
✍ Scribed by Berta Fiszer-Szafarz; David Szafarz; Alba Guevara de Murillo
- Book ID
- 102628730
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 415 KB
- Volume
- 110
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
A fluorometric method using 3,Sdiaminobenzoic acid for DNA determination in tissues, cultured cells, nucleated blood cells, and yeast cells is described. The method is general, simple, and rapid, and does not require prior DNA extraction, since tissue is directly soh&ilized in Triton X-100 and ammonia. The procedure is highly sensitive, and is able to measure rather accurately as little as 10 ng of DNA. It is applicable to all types of DNA structure. The DNA content determined in various tissues and cells was: 2.50 mg/g fresh rat liver, 3.32 mg/g rat diethylnitrosamine-induced hepatoma, 2.49 mg/g fresh mouse liver, 8.76 &loB human leukocytes, 3.37 cLg/loB chicken fibroblasts, 2.97 wdlO@ haploid yeast cells, and 2.84 cLg/loS haploid yeast protoplasts.
Animals and Cell Types
Rat livers were taken from male adult Sprague-Dawley animals which had been