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A gene controlling segregation of the Bacillus subtilis plasmid pC194

✍ Scribed by Alonso, Juan Carlos ;Trautner, Thomas A.

Publisher
Springer
Year
1985
Tongue
English
Weight
714 KB
Volume
198
Category
Article
ISSN
0026-8925

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✦ Synopsis

Plasmid pC194-1, a mutant of pC194, and chimeric derivatives of pC194-1 are segregationally unstable in B. subtilis. Such instability could be enhanced by exposure of pC194-1-carrying cells to methyl methanesulfonate. pC194-1 is distinct from pC194 in the addition of two A:T base pairs within the previously defined D region of pC194. Complementation experiments between pC194-1 and other plasmids suggest that the mutation of pC194-1 interferes with the production of a diffusible gene product required for plasmid maintenance.

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