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A gene-cloning system for Kluyveromyces lactis and isolation of a chromosomal gene required for killer toxin production

✍ Scribed by Dr. X. J. Chen; M. Wésolowski-Louvel; C. Tanguy-Rougeau; M. M. Bianchi; L. Fabiani; M. Saliola; C. Falcone; L. Frontali; H. Fukuhara

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
627 KB
Volume
28
Category
Article
ISSN
0233-111X

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✦ Synopsis

A transformation system derived from the circular plasmid p K D l has been developed for Kluyverom y m krclie. The principle is essentially equivalent to that of the 2 (r/5aecharomycea cereuiaim transformation system. The main features of the system are presented. Using a pKD1-based DNA bank of K . lactia, the K E X l gene involved in the killer system was isolated by complementation.

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✍ Leibowitz, Michael J. ;Wickner, Reed B. 📂 Article 📅 1978 🏛 Springer 🌐 English ⚖ 682 KB

Mutations in the pet18 gene of Saccharomyces cerevisiae (formerly denoted pets) confer three phenotypes on mutant strains: (i) inability to respire (petite), (ii) inability to maintain the double-stranded RNA killer plasmid (sensitive), and (iii) temperature sensitivity for growth. We find that pet1